In silico PCR amplification

Sequence

Primer 11    5'- -3'
Primer 21    5'- -3'

Allow one mismatch 2

Maximum lenght of bands
  nucleotides

1 A,T,G,C and N are allowed; A+T+G+C must be 10 or more.
2 One mismatch is allowed in any position of primers.



Source code