Melting Temperature (Tm) Calculation

Primer (6-50 bases):
LENGTH                   38
C+G%                     34.2
Upper Molecular weight:  11577.735
Lower Molecular weight:  11857.945
Basic Tm
Degenerated nucleotides are allowed
Base-Stacking Tm
Degenerated nucleotides are NOT allowed
Primer concentration: nM
Salt concentration: mM
Mg2+ concentration: mM
Source code is freely downloable at biophp.org